The holding temperature of blood during a delay to processing can affect serum and plasma protein measurements

Correct blood-borne biomarkers are hunted for analysis, prognosis and remedy stratification. Constant dealing with of blood is important for significant knowledge interpretation, nonetheless, delays throughout processing are sometimes unavoidable. We investigated the results of instantly inserting blood samples on ice versus room temperature for 1 h (reference protocol), and holding samples on ice versus room temperature throughout a three h delay to processing. Utilizing Luminex multi-plex assays to evaluate cytokines (n = 29) and diabetes-associated proteins (n = 15) in wholesome topics, we noticed that inserting blood samples instantly on ice decreased the serum ranges of a number of cytokines, together with PAI-1, MIP1-β, IL-9, RANTES and IL-8.

Throughout a delay to processing, some analytes, e.g. leptin and insulin, confirmed little change in serum or plasma values. Nevertheless, for about half of the analytes studied, a delay, whatever the holding temperature, altered the measured ranges in comparison with the reference protocol. Results differed between serum and plasma and for some analytes the course of change in degree assorted throughout people. The optimum holding temperature for samples throughout a delay was analyte-specific. In conclusion, deviations from protocol can result in important modifications in blood analyte ranges.

The place attainable, protocols for blood dealing with must be pre-determined in an analyte-specific method. Many sufferers fail to answer EGFR-targeted therapeutics, and customized diagnostics is required to determine putative responders. We investigated 1630 colorectal and lung squamous carcinomas and 1357 regular lung and colon samples and noticed big variation in EGFR pathway activation in each cancerous and wholesome tissues, irrespectively on EGFR gene mutation standing. We investigated whether or not human blood serum can have an effect on squamous carcinoma cell progress and EGFR drug response.

We show that human serum antagonizes the results of EGFR-targeted medicine erlotinib and cetuximab on A431 squamous carcinoma cells by rising IC50 by about 2- and 20-fold, respectively. The consequences on clonogenicity assorted considerably throughout the person serum samples in each experiment, with as much as 100% variations. EGF focus might clarify many results of blood serum samples, and EGFR ligands-depleted serum confirmed lesser impact on drug sensitivity.

In the direction of growth of a novel screening technique for figuring out Alzheimer’s illness danger: Raman spectroscopy of blood serum and machine studying

There’s an pressing medical want for a quick and efficient technique for diagnosing Alzheimer’s illness (AD). The identification of AD in its most preliminary phases, at which level remedy might present most therapeutic advantages, just isn’t solely prone to decelerate illness development however to additionally doubtlessly present a treatment. Nevertheless, present medical detection is sophisticated and requires a mixture of a number of strategies based mostly on important medical manifestations attributable to widespread neurodegeneration. As such, Raman spectroscopy with machine studying is investigated as a novel various technique for detecting AD in its earliest phases.

Right here, blood serum obtained from rats fed both an ordinary weight loss plan or a high-fat weight loss plan was analyzed. The high-fat weight loss plan has been proven to provoke a pre-AD state. Partial least squares discriminant evaluation mixed with receiver working attribute curve evaluation was capable of separate the 2 rat teams with 100% accuracy on the donor degree throughout exterior validation. Though additional work is important, this analysis suggests there’s a potential for Raman spectroscopy for use sooner or later as a profitable technique for figuring out AD early on in its development, which is important for efficient remedy of the illness.

This was a randomized crossover medical trial with 44 obese or overweight women. After a 2-wk run-in interval, women have been randomly assigned to both intervention or management teams. Members within the intervention group have been requested to acquire 50% of their grain servings from entire grain meals every day for six wk. These within the management group have been requested to not devour any of those meals. A 4-wk washout interval was utilized following which contributors have been crossed over to the alternate arm. Fasting blood samples have been taken earlier than and after every part of examine.

The holding temperature of blood during a delay to processing can affect serum and plasma protein measurements

Affiliation of Serum Testosterone and Luteinizing Hormone With Blood Strain and Danger of Cardiovascular Illness in Center-Aged and Aged Males

Background The age-related decline in testosterone ranges is regarded as of nice significance for male getting old and cardiovascular ailments. Nevertheless, knowledge are controversial on whether or not irregular intercourse hormones are linked to the presence of cardiovascular ailments and it’s also unsure how blood strain modifies the affiliation between testosterone ranges and main cardiovascular ailments. Strategies and Outcomes It is a multicenter, population-based, cross-sectional examine of 6296 males performed between 2013 and 2016. Primary info and medical signs have been obtained by questionnaires.

Blood strain and plasma ranges of complete testosterone, intercourse hormone-binding globulin, luteinizing hormone, and free testosterone have been decided in males in a multistage random, cluster sampling in 6 provinces of China. There have been 5786 Chinese language males (imply [SD] age 55.0 [10.1] years) included after exclusion standards have been utilized; 37.2% (2150) of them have been recognized with hypertension. Whole testosterone, free testosterone, and intercourse hormone-binding globulin have been inversely related to the prevalence of hypertension.

Horse Serum Albumin

30R-3300 10 mg
EUR 257
Description: Purified native Horse Serum Albumin

Horse Serum (Sterile)

20-abx800049
  • EUR 829.00
  • EUR 1080.00
  • EUR 578.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Serum (Sterile)

abx800049-1L 1 L
EUR 1316
  • Shipped within 1-5 working days.

Doner Horse Serum

F0605-050 500ml
EUR 151

Normal Goat Serum

88-B9999G000-S0 10 ml
EUR 133
Description: Control Normal Goat serum

Normal Canine Serum

88-NC25 100 ml
EUR 606
Description: Normal Canine Serum

Normal Goat Serum

88-NG22 500 ml
EUR 30
Description: Control Normal Goat Serum

Normal Goat Serum

88-NG22S 500 ml
EUR 191
Description: Control Normal Goat Serum

Normal Mouse Serum

88-NM35 50 ml
EUR 516
Description: Normal Mouse Serum

Normal Pig Serum

88-NP 500 ml
EUR 243
Description: Normal Pig Serum collected from pigs in the USA

Normal Rabbit Serum

88-NR50 100 ml
EUR 150
Description: Normal Rabbit Serum

Normal Rat Serum

88-NR51 50 ml
EUR 599
Description: Normal Rat Serum

Normal Sheep Serum

88-NS55 100 ml
EUR 126
Description: Normal Sheep Serum

Normal Mouse Serum

88R-1002 5 ml
EUR 203
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rabbit Serum

88R-1004 10 ml
EUR 206
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Goat Serum

88R-1006 10 ml
EUR 182
Description: Normal Goat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Sheep Serum

88R-1008 10 ml
EUR 182
Description: Normal Sheep Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rat Serum

88R-1010 5 ml
EUR 202
Description: Normal Rat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Chicken Serum

88R-1014 5 ml
EUR 176
Description: Normal Chicken Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Donkey Serum

88R-1022 10 ml
EUR 182
Description: Normal Donkey Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Pig Serum

88R-1023 2 ml
EUR 116
Description: Normal Pig (Swine) Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Cat Serum

88R-1026 10 ml
EUR 250
Description: Normal Cat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Dog Serum

88R-1028 10 ml
EUR 251
Description: Normal Dog Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Bovine Serum

88R-B001 100 ml
EUR 165
Description: Control Normal Bovine Serum

Normal Donkey Serum

88R-D001 100 ml
EUR 179
Description: Control Normal Donkey Serum

Normal Donkey Serum

88R-D003 10 ml
EUR 149
Description: Control Normal Donkey Serum

Normal Goat Serum

88R-NG001 50 ml
EUR 117
Description: Normal Goat Serum for use as a control or blocking reagent

Normal Pig Serum

88R-P004 1 liter
EUR 673
Description: Control Normal Pig Serum

Normal Sheep Serum

88R-S002 100 ml
EUR 165
Description: Control Normal Sheep Serum

Normal Rabbit Serum

89R-RM001 20 mg
EUR 392
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RM002 5 ml
EUR 241
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RR001 20 mg
EUR 349
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Rabbit Serum

89R-RR002 5 ml
EUR 241
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Goat Serum

9067 50 ml
EUR 100.75
Description: Normal Goat Serum

Normal Human Serum

90R-1002 10 ml
EUR 254
Description: Normal Human Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Bovine Serum

abx098263-100ml 100 ml
EUR 258
  • Shipped within 5-10 working days.

Horse Serum Albumin antibody

70R-15104 100 ug
EUR 327
Description: Rabbit polyclonal Horse Serum Albumin antibody

Horse Serum (Non-Sterile)

20-abx800048
  • EUR 648.00
  • EUR 829.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Serum (Non-Sterile)

abx800048-1L 1 L
EUR 1080
  • Shipped within 1-5 working days.

Normal goat serum(blocking)

AR1009 100mL
EUR 131

Normal Guinea Pig Serum

88-NP25 50 ml
EUR 220
Description: Normal Guinea Pig Serum

Normal Guinea Pig Serum

88R-1016 5 ml
EUR 142
Description: Normal Guinea Pig Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Syrian Hamster Serum

88R-1018 5 ml
EUR 173
Description: Normal Syrian Hamster Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Donkey Serum filtered

88R-D1052 100 ml
EUR 223
Description: Control Normal Donkey Serum filtered

Normal Human Serum (filtered)

90-1013 100 ml
EUR 548
Description: Human Serum Off The Clot from Normal Donors

Buffered Normal Goat Serum

9066 50 ml
EUR 164.55
Description: Buffered Normal Goat Serum

Horse Serum Sterile, Non-Hemolyzed

39317-5 100mL
EUR 138
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Whole blood is collected from both male and female healthy horses living in a herd and are 1+ years of age. The blood is refrigerated within 6 hours after collection and is processed within 24 hours and frozen at -20°C. Filtered through a 0.22 micron filter.

Horse Serum Sterile, Non-Hemolyzed

39321-1 500mL
EUR 161
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Whole blood is collected from both male and female healthy horses living in a herd and are 1+ years of age. The blood is refrigerated within 6 hours after collection and is processed within 24 hours and frozen at -20°C. Filtered through a 0.22 micron filter.

Horse Serum Sterile, Non-Hemolyzed

39321-2 3L
EUR 429
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Whole blood is collected from both male and female healthy horses living in a herd and are 1+ years of age. The blood is refrigerated within 6 hours after collection and is processed within 24 hours and frozen at -20°C. Filtered through a 0.22 micron filter.

Horse Serum, Sterile, Heat inactivated

39324-5 3L
EUR 468
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Blood is aseptically collected from the donor herd horses. The blood is refrigerated within 8 hours of collection and processed within 48 hours after which it is stored at -20°C. The serum is thawed and passed through a 0.22 micron filter, bottled and stored at -20°C. The sterile serum is later thawed, heat inactivated at 56°C for 30 minutes, and returned to -20°C.

Horse Serum Albumin antibody (biotin)

60R-1153 100 ug
EUR 327
Description: Rabbit polyclonal Horse Serum Albumin antibody (biotin)

Horse Serum Albumin antibody (FITC)

60R-1154 100 ug
EUR 327
Description: Rabbit polyclonal Horse Serum Albumin antibody (FITC)

Horse Serum Albumin antibody (HRP)

60R-1155 100 ug
EUR 327
Description: Rabbit polyclonal Horse Serum Albumin antibody (HRP)

Horse Serum amyloid A (SAA1)

1-CSB-YP020656HO
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 14.3 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Horse Serum amyloid A(SAA1) expressed in Yeast

Horse Serum Albumin Polyclonal Antibody

A57536 100 µg
EUR 570.55
Description: Ask the seller for details

Normal Rabbit Serum (Blocking Solution)

AR1010 100mL (for 10000-15000 assays)
EUR 131

Normal Rat Serum (Heat Inactitvated)

88R-1032 500 ml
EUR 597
Description: Sterile Filtered Heat Inactitvated Normal Rat Serum

Pre-immune normal rabbit serum

NRS01 200 µl
EUR 299

Horse Serum Sterile, Non-hemolized, Delipidized

38002-1 500mL
EUR 135
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Whole blood is collected from both male and female healthy horses living in a herd and are 1+ years of age. The blood is refrigerated within 6 hours after collection and is processed within 24 hours and frozen at -20°C. The serum is then thawed in a cool water bath. First the lipids are removed, then the serum is filtered through a 0.22 micron filter. The obtained product is bottled and frozen at -20 ºC.

Horse Serum Non-Sterile, Non-Hemolyzed

39321-3 500mL
EUR 129
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Whole blood is collected from both male and female healthy horses living in a herd and are 1+ years of age. The blood is refrigerated within 6 hours after collection and is processed within 24 hours and frozen at -20°C.

Horse Serum amyloid A protein (SAA1)

1-CSB-EP020656HO
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 16.3 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Horse Serum amyloid A protein(SAA1) expressed in E.coli

Horse Serum amyloid A protein (SAA1)

1-CSB-EP020656HOb0
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 15.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Horse Serum amyloid A protein(SAA1) expressed in E.coli

Horse Serum Amyloid A (SAA) Protein

abx670163-01mg 0.1 mg
EUR 954
  • Shipped within 1 week.

Normal Bovine Serum (Protease/IgG free)

88R-1012 10 ml
EUR 182
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rat serum (non-immunized) Wistar

NRTW-1 1 ml
EUR 80

Normal Rat serum (non-immunized) Wistar

NRTW-5 5 ml
EUR 164

Horse Serum Amyloid A (SAA) ELISA Kit

20-abx258995
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Horse SAA(Serum Amyloid A)ELISA Kit

EHS0048 96T
EUR 567.6
  • Detection range: 0.156-10 ug/ml
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Horse;Sensitivity: 0.094 ug/ml

Horse Serum Amyloid A(SAA)ELISA Kit

CSB-E15951Hs-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Horse Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Horse Serum Amyloid A(SAA)ELISA Kit

1-CSB-E15951Hs
  • EUR 703.00
  • EUR 4843.00
  • EUR 2570.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Horse Serum Amyloid A(SAA) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Horse Serum Amyloid A (SAA) CLIA Kit

20-abx496579
  • EUR 8709.00
  • EUR 4638.00
  • EUR 1067.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.

Horse Serum Albumin Polyclonal Antibody, Biotin Conjugated

A57533 100 µg
EUR 570.55
Description: reagents widely cited

Horse Serum Albumin Polyclonal Antibody, FITC Conjugated

A57534 100 µg
EUR 570.55
Description: Ask the seller for details

Horse Serum Albumin Polyclonal Antibody, HRP Conjugated

A57535 100 µg
EUR 570.55
Description: The best epigenetics products

Horse control serum (non-immunized) Mixed breed

NHOS-10 10 ml
EUR 103

Horse control serum (non-immunized) Mixed breed

NHOS-100 100 ml
EUR 225

Horse control serum (non-immunized) Mixed breed

NHOS-500 500 ml
EUR 408

Horse Serum Amyloid A ELISA Kit (SAA)

RK00770 96 Tests
EUR 573

Horse Serum Amyloid A (SAA) ELISA Kit

SEA885Eq-10x96wellstestplate 10x96-wells test plate
EUR 5778.7
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Horse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse serum Amyloid A (SAA) in serum, plasma and other biological fluids.

Horse Serum Amyloid A (SAA) ELISA Kit

SEA885Eq-1x48wellstestplate 1x48-wells test plate
EUR 563.54
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Horse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse serum Amyloid A (SAA) in serum, plasma and other biological fluids.

Horse Serum Amyloid A (SAA) ELISA Kit

SEA885Eq-1x96wellstestplate 1x96-wells test plate
EUR 762.2
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Horse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse serum Amyloid A (SAA) in serum, plasma and other biological fluids.

Horse Serum Amyloid A (SAA) ELISA Kit

SEA885Eq-5x96wellstestplate 5x96-wells test plate
EUR 3129.9
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Horse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse serum Amyloid A (SAA) in serum, plasma and other biological fluids.

Horse Serum Amyloid A (SAA) ELISA Kit

4-SEA885Eq
  • EUR 5829.00
  • EUR 3080.00
  • EUR 763.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Serum Amyloid A elisa. Alternative names of the recognized antigen: SA-A
  • SAA1
  • PIG4
  • TP53I4
  • Amyloid protein A
  • Amyloid fibril protein AA
  • Serum amyloid A-1 protein
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Horse Serum Amyloid A (SAA) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

7078 10 PIPET 10ML STER MULTI PACK

7078-10N 120/pk
EUR 268
Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line

7078B PIPET SHRTY 50ML MULTI-PACK, STER

7078B-50 25/pk
EUR 186
Description: Disposable Pipets; Disposable Pipets, Serological, Misc

ELISA kit for Horse SAA (Serum Amyloid A)

ELK8283 1 plate of 96 wells
EUR 526
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid A (SAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serum Amyloi
  • Show more
Description: A sandwich ELISA kit for detection of Serum Amyloid A from Horse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Bactrian camel (Camelus bactrianus; normal, non-immune) serum

NBCS-1 1 ml
EUR 286

7077B PIPET SHRTY 50ML STER, DISP, IND WR

7077B-50 25/pk
EUR 186
Description: Disposable Pipets; Disposable Pipets, Serological, Misc

Normal Alpaca serum (non-immunized, control, adult mixed sex)

NAPS-1 1 ml
EUR 225

Normal Llama serum (non-immunized, control, adult mixed sex)

NLLS-1 1 ml
EUR 128

Horse control serum (non-immunized) delipidized, heat inactivate Mixed breed

NHOSH-10 10 ml
EUR 128

Horse control serum (non-immunized) delipidized, heat inactivate Mixed breed

NHOSH-100 100 ml
EUR 286

Horse control serum (non-immunized) delipidized, heat inactivate Mixed breed

NHOSH-500 500 ml
EUR 469

Recombinant Horse Serum amyloid A protein Protein, His, E.coli-100ug

QP6647-ec-100ug 100ug
EUR 707

Recombinant Horse Serum amyloid A protein Protein, His, E.coli-10ug

QP6647-ec-10ug 10ug
EUR 326

Recombinant Horse Serum amyloid A protein Protein, His, E.coli-1mg

QP6647-ec-1mg 1mg
EUR 2303

Recombinant Horse Serum amyloid A protein Protein, His, E.coli-200ug

QP6647-ec-200ug 200ug
EUR 1115

Recombinant Horse Serum amyloid A protein Protein, His, E.coli-500ug

QP6647-ec-500ug 500ug
EUR 1514

Recombinant Horse Serum amyloid A protein Protein, His, E.coli-50ug

QP6647-ec-50ug 50ug
EUR 435

Recombinant Horse Serum amyloid A protein Protein, His, Yeast-100ug

QP6647-ye-100ug 100ug
EUR 788

Recombinant Horse Serum amyloid A protein Protein, His, Yeast-10ug

QP6647-ye-10ug 10ug
EUR 362

Recombinant Horse Serum amyloid A protein Protein, His, Yeast-1mg

QP6647-ye-1mg 1mg
EUR 2747

Recombinant Horse Serum amyloid A protein Protein, His, Yeast-200ug

QP6647-ye-200ug 200ug
EUR 1260

Recombinant Horse Serum amyloid A protein Protein, His, Yeast-500ug

QP6647-ye-500ug 500ug
EUR 1804

Recombinant Horse Serum amyloid A protein Protein, His, Yeast-50ug

QP6647-ye-50ug 50ug
EUR 480

Horse IgM

31C-CH0813 1 mg
EUR 365
Description: Purified Horse IgM

Horse IgG

E61I024 1mg
EUR 343

Horse Glucose

QY-E120080 96T
EUR 478

Horse GGTP

QY-E120082 96T
EUR 478

Horse Lactate

QY-E120087 96T
EUR 478

Horse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Protein

20-abx166393
  • EUR 759.00
  • EUR 300.00
  • EUR 2388.00
  • EUR 913.00
  • EUR 537.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Polyclonal Antibody (Horse)

4-PAA116Eq01
  • EUR 262.00
  • EUR 2747.00
  • EUR 679.00
  • EUR 331.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RANTES (Ser24~Ser91)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Horse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

Rat Bone PrimaCell: Normal Osteoblasts Growth Supplements with Serum (for 500 ml medium)

9-26010 1 Set Ask for price

Rat Skin PrimaCell2: Normal Melanocytes Growth Supplements with Serum (for 500 ml medium)

9-26083 1 Set Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-26095 1 Set Ask for price

Mouse Bone PrimaCell: Normal Osteoblasts Growth Supplements with Serum (for 500 ml medium)

9-33009 1 Set Ask for price

Mouse Kidney PrimaCell3: Normal Podocytes Growth Supplements with Serum (for 500 ml medium)

9-33058 1 Set Ask for price

Mouse Skin PrimaCell2: Normal Melanocytes Growth Supplements with Serum (for 500 ml medium)

9-33086 1 Set Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-33100 1 Set Ask for price

Human Bone PrimaCell: Normal Osteoblasts Growth Supplements with Serum (for 500 ml medium)

9-47012 1 Set Ask for price

Human Fat PrimaCell2: Normal Preadipocytes Growth Supplements with Serum (for 500 ml medium)

9-47063 1 Set Ask for price

Human Skin PrimaCell2: Normal Melanocytes Growth Supplements with Serum (for 500 ml medium)

9-47102 1 Set Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-47121 1 Set Ask for price

Horse Gamma Globulin

29305-1 1g
EUR 129
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Horse gamma globulin is purified from serum obtained from normal healthy animals (male and female) by using various fractionation procedures. It is in lyophilized powder form with purity of 95% or more. Protein by weight: O.D. 280 nm E1%1.39: ? 85% - Residual salts, lipids, etc. comprise the rest of the weight.

Horse Gamma Globulin

29305-2 10g
EUR 454
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Horse gamma globulin is purified from serum obtained from normal healthy animals (male and female) by using various fractionation procedures. It is in lyophilized powder form with purity of 95% or more. Protein by weight: O.D. 280 nm E1%1.39: ? 85% - Residual salts, lipids, etc. comprise the rest of the weight.

Horse Gamma Globulin

29305-5 500g
EUR 417
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Horse gamma globulin is purified from serum obtained from normal healthy animals (male and female) by using various fractionation procedures. It is in lyophilized powder form with purity of 95% or more. Protein by weight: O.D. 280 nm E1%1.39: ? 85% - Residual salts, lipids, etc. comprise the rest of the weight.

Horse RBC antibody

20R-RR023 20 mg
EUR 327
Description: Rabbit polyclonal Horse RBC antibody

Horse RBC antibody

20R-RR024 5 mg
EUR 554
Description: Rabbit polyclonal Horse RBC antibody

Horse IgG (FITC)

65C-CH0802 1 mg
EUR 169
Description: Purified Horse IgG FITC conjugate

Horse IgG (HRP)

65C-CH0803 1 mg
EUR 227
Description: Purified Horse IgG HRP conjugate

Recombinant Horse Leptin

7-01174 20µg Ask for price

Recombinant Horse Leptin

7-01175 100µg Ask for price

Recombinant Horse Leptin

7-01176 1mg Ask for price

Horse IgG (Fc)

31C-CH0804 1 mg
EUR 314
Description: Purified Horse IgG (Fc)

Horse IgG (Fab)

31C-CH0810 2 mg
EUR 340
Description: Purified Horse IgG (Fab)

Horse Ig fraction

31R-1005 10 mg
EUR 114
Description: Horse Ig fraction control or blocking reagent

Horse Ig fraction

31R-1029 10 mg
EUR 114
Description: Purified Horse Ig fraction for use as a control or blocking reagent

Horse IgG protein

31R-1055 10 mg
EUR 187
Description: Purified native Horse IgG protein

Horse Erythropoietin (EPO)

1-CSB-EP007743HO
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 34.3 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Horse Erythropoietin(EPO) expressed in E.coli

Horse Latherin (LATH)

1-CSB-YP306756HO
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 24.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Horse Latherin(LATH) expressed in Yeast

Recombinant Horse TNFA

P0277 100ug
EUR 522.36
  • Formulation: pH7.4, Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose
  • Reconstitution: Sterile distilled water
  • Purity: Greater than 95% by SDS-PAGE gel analyses
  • Uniprot ID: P29553
Description: Recombinant Horse protein for TNFA

Horse thyroxine(T4)

QY-E120089 96T
EUR 478

Horse Cortisol(COR)

QY-E120091 96T
EUR 478

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Polyclonal Antibody (Horse), APC

4-PAA116Eq01-APC
  • EUR 368.00
  • EUR 3599.00
  • EUR 993.00
  • EUR 472.00
  • EUR 229.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RANTES (Ser24~Ser91)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Horse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). This antibody is labeled with APC.

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Polyclonal Antibody (Horse), Biotinylated

4-PAA116Eq01-Biotin
  • EUR 328.00
  • EUR 2697.00
  • EUR 786.00
  • EUR 404.00
  • EUR 226.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RANTES (Ser24~Ser91)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Horse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). This antibody is labeled with Biotin.

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Polyclonal Antibody (Horse), Cy3

4-PAA116Eq01-Cy3
  • EUR 449.00
  • EUR 4757.00
  • EUR 1283.00
  • EUR 588.00
  • EUR 264.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RANTES (Ser24~Ser91)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Horse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). This antibody is labeled with Cy3.

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Polyclonal Antibody (Horse), FITC

4-PAA116Eq01-FITC
  • EUR 314.00
  • EUR 2899.00
  • EUR 814.00
  • EUR 397.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RANTES (Ser24~Ser91)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Horse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). This antibody is labeled with FITC.

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Polyclonal Antibody (Horse), HRP

4-PAA116Eq01-HRP
  • EUR 335.00
  • EUR 3135.00
  • EUR 877.00
  • EUR 426.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RANTES (Ser24~Ser91)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Horse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). This antibody is labeled with HRP.

Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Polyclonal Antibody (Horse), PE

4-PAA116Eq01-PE
  • EUR 314.00
  • EUR 2899.00
  • EUR 814.00
  • EUR 397.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RANTES (Ser24~Ser91)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Horse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). This antibody is labeled with PE.

Rat Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Supplements with Serum (for 500 ml medium)

9-26007 1 Set Ask for price

Rat Brain PrimaCell1: Normal Brain Fibroblasts Growth Supplements with Serum (for 500 ml medium)

9-26011 1 Set Ask for price

Rat Brain PrimaCell6: Normal Meningeal Cells Growth Supplements with Serum (for 500 ml medium)

9-26017 1 Set Ask for price

Rat Brain PrimaCell7: Normal Nerve Astrocytes Growth Supplements with Serum (for 500 ml medium)

9-26018 1 Set Ask for price

Rat Brain PrimaCell8: Normal Nerve Microglia Growth Supplements with Serum (for 500 ml medium)

9-26019 1 Set Ask for price

Rat Brain PrimaCell9: Normal Neuron Cell Growth Supplements with Serum (for 500 ml medium)

9-26020 1 Set Ask for price

Rat Cartilage PrimaCell: Normal Articular Cartilage Growth Supplements with Serum (for 500 ml medium)

9-26023 1 Set Ask for price

Rat Cervix PrimaCell2: Normal Cervical Fibroblasts Growth Supplements with Serum (for 500 ml medium)

9-26025 1 Set Ask for price

Rat Embryo PrimaCell4: Normal Trophoblast Cells Growth Supplements with Serum (for 500 ml medium)

9-26034 1 Set Ask for price

Rat Eye PrimaCell3: Normal Corneal Fibroblasts Growth Supplements with Serum (for 500 ml medium)

9-26040 1 Set Ask for price

Rat Fat PrimaCell1: Normal Adipose Cells Growth Supplements with Serum (for 500 ml medium)

9-26043 1 Set Ask for price

Rat Heart PrimaCell2: Normal Atrial Myocytes Growth Supplements with Serum (for 500 ml medium)

9-26047 1 Set Ask for price

Rat Heart PrimaCell3: Normal Heart Fibroblasts Growth Supplements with Serum (for 500 ml medium)

9-26048 1 Set Ask for price

Age >65 years or physique mass index ≥24 negatively impacted the inverse correlation between testosterone ranges and hypertension, whereas smoking and household historical past of hypertension strengthened the correlation. In contributors with grade 2 hypertension, complete testosterone was positively related to the presence of stroke, and luteinizing hormone was additionally positively correlated with cardiovascular and cerebrovascular ailments. Conclusions Decrease complete testosterone may very well be a promising danger marker for prevalent hypertension. Each high and low ranges of testosterone are related to higher cardiovascular danger. Major hypogonadism could also be a danger marker for main cardiovascular ailments in males with extreme hypertension.

Effects of Clove Oil as a Euthanasia Agent on Blood Collection Efficiency and Serum Cortisol Levels in Danio rerio.

Effects of Clove Oil as a Euthanasia Agent on Blood Collection Efficiency and Serum Cortisol Levels in Danio rerio.

The zebrafish is an important laboratory animal model for biomedical research and are increasingly being used for behavioral neuroscience. Tricaine methanesulfonate (MS222) is the standard agent used for euthanasia of zebrafish.

However, recent studies show that zebrafish behavior MS222 possible hostility, and clove oil may be a possible alternative. In this study, we compared the effects MS222 or clove oil as euthanasia agents in zebrafish on the volume of blood was collected and the serum levels of cortisol.

A larger amount of serum can be collected and a lower serum levels of cortisol present at euthanasia fish with clove oil as compared with equipotent doses of MS222. Euthanasia with clove oil does not blunt the expected elevation of serum cortisol levels induced by acute stress premortem.

According to our findings, clove oil is a fast-acting agent which minimizes cortisol responses to euthanasia in zebrafish and allows the collection of a large volume of blood postmortem. These results represent a significant improvement in the method of euthanasia for zebrafish.

Effects of Clove Oil as a Euthanasia Agent on Blood Collection Efficiency and Serum Cortisol Levels in Danio rerio.
Effects of Clove Oil as a Euthanasia Agent on Blood Collection Efficiency and Serum Cortisol Levels in Danio rerio.

Serum C-reactive protein and white blood cell level as markers of successful percutaneous drainage of acute sterile peripancreatic fluid collection.

BACKGROUND
Percutaneous drainage is not a method of therapy is widely used recently to evacuate sterile peripancreatic fluid collections in patients with severe acute pancreatitis.However, many clinical studies have shown positive effects.

OBJECTIVE
We tested changes in laboratory parameters of serum C-reactive protein (CRP), complement factor 3-4 (C 3-4), tumor necrosis factor a (TNF-a), amylase, lipase and white blood cell (WBC) count in patients treated with percutaneous drainage.


METHOD
10 patients with severe acute pancreatitis with peripancreatic fluid collections were monitored.Laboratory parameters and the amount of fluid drained was measured on the 1st, 5th and 10th day. Statistical analysis was performed using Statistics for Windows (Version 7.0) software. P values ​​less than 0.05 were considered statistically significant.

RESULTS
We found a significant positive correlation between serum levels of CRP and WBC and drained fluid volume. We use this parameter as a marker of successful percutaneous drainage in the case of patients with severe acute pancreatitis complicated with sterile peripancreatic fluid.There no significant change in levels of C 3-4, tumor necrosis factor-Î + -, amylase and lipase.


CONCLUSION
Monitoring of serum CRP and WBC levels may be recommended for follow-up after percutaneous drainage of peripancreatic fluid.
BACKGROUND
CRP: C-reactive protein TNFÎ + -: Tumor Necrosis Factor a, C3-4: Complementary 3-4 WBC: White Blood Cells CT: Computed Tomography.

Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome.

Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome.

Background: Metabolomics is emerging as a valuable tool in the clinical sciences. However, one of the major challenges in clinical metabolomics is of limited use standardized guidelines for sample collection and handling. In this study, we conducted a pilot analysis of serum and plasma to determine the effects of processing time and collection tubes at the metabolome.

Methods: Blood was collected in three tubes: Vacutainer serum separator tube (SST, serum), EDTA (plasma) and P100 (plasma) and stored at 4 degrees for 0, 0.5, 1, 2, 4 and 24 hours prior to centrifugation. Compounds were extracted using liquid-liquid extraction to obtain a fraction of hydrophilic and hydrophobic and analyzed using liquid chromatography-mass spectrometry.

The difference between blood collection tubes and sample processing time is evaluated (ANOVA, Bonferroni FWER ≤ 0.05 and ANOVA, Benjamini Hochberg FDR ≤ 0.1, respectively). Results: Among the serum and plasma tubes 93.5% of the compound overlaps, 382 unique compounds for serum and one unique compound to the plasma. There are 46, 50 and 86 compounds is affected by the processing time in the SST, EDTA tube and P100, respectively, including many lipids.

In contrast, 496 242 hydrophilic and hydrophobic compounds different from a collection tube. Forty-five different chemical classes include alcohols, sugars, amino acids and lipids prenol influenced by the choice of blood collection tubes.

Conclusion: Our results indicate that the choice of blood collection tubes have a significant impact on the metabolites detected and their overall abundance. Perhaps surprisingly, the variation in sample processing time has less effect than the collection tube; However, a larger sample size is needed to confirm this.

Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome.
Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome.

Therapeutic antibiotic serum concentrations by two blood collection methods within the pediatric patient: A comparative effectiveness trial.

venipunctures repeatedly and fingersticks to confirm serum drug concentration causing pain and discontent for pediatric patients and their families. In many organizations, the standard of care to obtain therapeutic serum drug concentration by peripheral venipuncture or fingerstick capillary, even when the patient has any peripheral intravenous catheter (PIV) or a central venous catheter (CVC).

The main objective of this study was to assess the agreement between serum tobramycin / vancomycin concentrations were collected from CVC or PIV, compared to venipuncture or fingerstick. Among patients hospitalized children (aged 3 months to 22 years), 36 paired blood samples were collected.

Through random serum vancomycin and tobramycin concentrations were compared between samples of peripheral intravenous or CVC, and venipuncture or fingerstick samples in the same patient.

A rigorous sampling protocol to obtain samples followed, including sample collection CVC / PIV before venipuncture or fingerstick, less than 2 minutes between collections of samples from different sites, and based on the volume of waste flush and strict protocol. corresponding correlation coefficient showed a substantial agreement between the CVC / PIV and venipuncture / fingerstick concentration to vancomycin (n = 17) and tobramycin (n = 19) (Rc = 0.982 for both).

Bland-Altman analysis showed overall good agreement between methods in these subjects and minimal bias. As a result, use and waste protocols flush lumen volume based-, children with venipunctures catheter may not require additional and / or fingersticks confirmation of drug concentrations while hospitalized, improve service quality and patient satisfaction.

Switching from serum to plasma: Implementation of BD Vacutainer® Barricor™ Plasma Blood Collection Tubes improves sample quality and laboratory turnaround time.

Switching from serum to plasma: Implementation of BD Vacutainer® Barricor™ Plasma Blood Collection Tubes improves sample quality and laboratory turnaround time.

For blood, most standard 24/7 (immuno) chemical parameters measured either in serum or lithium-heparin plasma. Standard tube gel serum and plasma have their flaws when timely analysis required high quality results.

Serum require the freezing time and disruption of bubbles in the plasma gel and hydrophobic analyte adsorption into the gel layer is potentially harmful high-quality results from gel lithium heparin tube.

We sought to evaluate the impact of the BD Vacutainer® Barricor ™ Tube (Barricor ™) in laboratory efficiency by measuring its effect on TAT and quality of the samples, as well as evaluating opportunities potential costs resulting from an increase in the sample data extracted quality.

TAT and remediation activities and was arrested for two 6 phases of the moon. Serum is used as the dominant matrix of the first stage and Barricor ™ plasma used in the second phase.Barricor ™ significantly reduced the median TAT, especially for routine sample-priority during peak hours.

TAT key performance indicators (percentage yield available within 90 minutes) increased to> 90% for STAT and routine priority samples. Conversion of gel serum, Barricor ™ reduces fibrin-related remediation activities from 2.3% to 0.4%. This resulted in a reduction in remediation-related costs of € 6010.47 during the study period.

By apply Barricor ™, we see a significant reduction in TAT and reduction of fibrin-related remediation time and cost, when compared to the dominant serum workflow. TAT improved opens up the possibility of consolidation to a single priority level, eliminating the need to use STAT priority level.

Switching from serum to plasma: Implementation of BD Vacutainer® Barricor™ Plasma Blood Collection Tubes improves sample quality and laboratory turnaround time.
Switching from serum to plasma: Implementation of BD Vacutainer® Barricor™ Plasma Blood Collection Tubes improves sample quality and laboratory turnaround time.

Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in serum and heparin plasma samples.

For blood, most standard 24/7 (immuno) chemical parameters measured either in serum or lithium-heparin plasma. Standard tube gel serum and plasma have their flaws when timely analysis required high quality results. Serum require the freezing time and disruption of bubbles in the plasma gel and hydrophobic analyte adsorption into the gel layer is potentially harmful high-quality results from gel lithium heparin tube.

We sought to evaluate the impact of the BD Vacutainer® Barricor ™ Tube (Barricor ™) in laboratory efficiency by measuring its effect on TAT and quality of the samples, as well as evaluating opportunities potential costs resulting from an increase in the sample data extracted quality.

TAT and remediation activities and was arrested for two 6 phases of the moon. Serum is used as the dominant matrix of the first stage and Barricor ™ plasma used in the second phase.

Barricor ™ significantly reduced the median TAT, especially for routine sample-priority during peak hours. TAT key performance indicators (percentage yield available within 90 minutes) increased to> 90% for STAT and routine priority samples. Conversion of gel serum, Barricor ™ reduces fibrin-related remediation activities from 2.3% to 0.4%.

This resulted in a reduction in remediation-related costs of € 6010.47 during the study period.By apply Barricor ™, we see a significant reduction in TAT and reduction of fibrin-related remediation time and cost, when compared to the dominant serum workflow. TAT improved opens up the possibility of consolidation to a single priority level, eliminating the need to use STAT priority level.

Bloodbanks

Automation in the bloodbank: things to think about.

Prevention of immune-mediated transfusion-related acute lung injury; from bloodbank to patient.

Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion related morbidity and mortality. Immune-mediated TRALI is caused by leucocyte and neutrophil antibodies in the transfused blood products that react with white blood cell antigens of the recipient, hereby inducing endothelial damage and lung injury. About two thirds of TRALI cases are thought to be immune-mediated. Both Human Leucocyte Antibodies (HLA Class I and II) and Human Neutrophil Antibodies (HNA) are involved in TRALI. Most antibodies result from allo-exposure of the blood donor, with multiparous donors having the highest incidence of antibodies. Detection of anti-leucocyte and anti-neutrophil antibodies is complex and many uncertainties still exist regarding the interpretation of the test results. In this review we discuss the evidence and effectiveness of measurements to prevent immune-mediated TRALI from a bloodbank and bedside perspective. From a bloodbank perspective various preventive measures have been implicated. In some countries bloodbanks have successfully implemented donor selection strategies, ranging from testing of allo-exposed donors for leucocyte antibodies to the exclusion of all females from donating high plasma volume products. Another strategy involves dilution of antibodies present by pooling of plasma donations of multiple donors. From a bedside view, the most important measure to prevent TRALI is to limit patients’ exposure to allogenic bloodproducts. Furthermore recognition and awareness of the syndrome need to be heightened among clinicians.


Variants in neuropeptide Y receptor 1 and 5 are associated with nutrient-specific food intake and are under recent selection in Europeans.

There is a large variation in caloric intake and macronutrient preference between individuals and between ethnic groups, and these food intake patterns show a strong heritability. The transition to new food sources during the agriculture revolution around 11,000 years ago probably created selective pressure and shaped the genome of modern humans. One major player in energy homeostasis is the appetite-stimulating hormone neuropeptide Y, in which the stimulatory capacity may be mediated by the neuropeptide Y receptors 1, 2 and 5 (NPY1R, NPY2R and NPY5R). We assess association between variants in the NPY1R, NPY2R and NPY5R genes and nutrient intake in a cross-sectional, single-center study of 400 men aged 40 to 80 years, and we examine whether genomic regions containing these genes show signatures of recent selection in 270 HapMap individuals (90 Africans, 90 Asians, and 90 Caucasians) and in 846 Dutch bloodbank controls. Our results show that derived alleles in NPY1R and NPY5R are associated with lower carbohydrate intake, mainly because of a lower consumption of mono- and disaccharides. We also show that carriers of these derived alleles, on average, consume meals with a lower glycemic index and glycemic load and have higher alcohol consumption. One of these variants shows the hallmark of recent selection in Europe. Our data suggest that lower carbohydrate intake, consuming meals with a low glycemic index and glycemic load, and/or higher alcohol consumption, gave a survival advantage in Europeans since the agricultural revolution. This advantage could lie in overall health benefits, because lower carbohydrate intake, consuming meals with a low GI and GL, and/or higher alcohol consumption, are known to be associated with a lower risk of chronic diseases.
[Tranfusion counseling].

In this article, we present transfusion counseling; its organization, actors, their formations and we deal with factual positions. Transfusion counseling needs better identification, tending to a homogeneous organization between every bloodbank centre.


Genomics and blood substitutes for 21st century Europe (“EuroBloodSubstitutes”).

Blood transfusion is a medical intervention practised throughout the world. Blood is a biologically active material that can transmit diseases (e.g., HIV/AIDS and, perhaps, vCJD). People are becoming increasingly concerned about blood safety, despite improved screening and processing. Consequently, they are reluctant to donate blood or receive transfusions. Such problems can be solved by the development and incorporation into transfusion practices of so-called “blood substitutes” to replace some blood uses. The EuroBloodSubstitutes Project is funded by the European Union Framework 6 Programme to develop a technological platform for producing novel haem proteins and blood substitute components using micro-organisms (bacteria, fungi, yeast) as “cell factories.” The Project will focus on bacteria (Escherichia coli), yeast (Pichia pastoris) and, longer-term, filamentous fungi (Aspergillus niger), all organisms used to synthesize commercially important products. The multi-centre Consortium consists of the Universities of (1) Nottingham (UK), (2) Essex (UK), (3) Denmark Technical (Denmark), (4) Lund (Sweden), (5) Milan (Italy), (6) Nancy (France), (7) Parma (Italy), (8) Rome “La Sapienza” (Italy), (9) Semmelweis (Hungary), together with (10) Alligator Bioscience, AB (Sweden), (11) LCC Engineering & Trading GmbH (Switzerland), (12) Scottish National Blood Transfusion Service (UK), and (13) Sanquin Bloodbank (The Netherlands). The EuroBloodSubstitutes Project will be informed by lay and professional stakeholders (e.g., clinicians, blood donors, patient groups, prescribers and policy makers). Outcomes of the Project are (1) the production of an information pack, decision aids and physician training aids, giving balanced overviews of the benefits and risks of transfusion of blood or potential substitutes, and (2) an interactive web site

(http//:www.eurobloodsubstitutes.com) for information dissemination. This will improve knowledge and address misunderstandings about transfusion issues in a climate of changing patient expectations on blood safety and benefits of blood substitutes.


From anhydrobiosis to freeze-drying of eukaryotic cells.

Using what has been learned from nature, it has become possible to stabilize biological structures, including intact cells, in the dry state. Stabilization of cells or tissues in the dried state is of considerable practical significance, as is described in this review. The need for stabilization of cells in the dried state is particularly urgent in bloodbanks, where proper storage of blood cells (platelets and erythrocytes) is still a major problem. Human blood platelets are stored in blood banks for 5 days, after which they are discarded according to Federal regulation. This short lifetime has led to a chronic shortage of platelets. We report here that platelets can be preserved by freeze-drying them with trehalose, a sugar found at high concentrations in organisms that naturally survive drying. We suggest that this finding will obviate the storage problem with platelets and that the principles established here may be extended to more complex eukaryotic cells.
Universal leukoreduction in Iceland validation, process control and quality control. Implications for smaller bloodbanks.

Serological detection of attenuated HIV-1 variants with nef gene deletions.

OBJECTIVE
To investigate whether members of a transfusion-linked cohort (the Sydney Bloodbank Cohort) infected with a nef-deleted strain of HIV-1 could be differentiated from individuals infected with wild-type strains of HIV-1 by characterizing the Nef antibody response of cohort members.