Effects of Clove Oil as a Euthanasia Agent on Blood Collection Efficiency and Serum Cortisol Levels in Danio rerio.

Effects of Clove Oil as a Euthanasia Agent on Blood Collection Efficiency and Serum Cortisol Levels in Danio rerio.

The zebrafish is an important laboratory animal model for biomedical research and are increasingly being used for behavioral neuroscience. Tricaine methanesulfonate (MS222) is the standard agent used for euthanasia of zebrafish.

However, recent studies show that zebrafish behavior MS222 possible hostility, and clove oil may be a possible alternative. In this study, we compared the effects MS222 or clove oil as euthanasia agents in zebrafish on the volume of blood was collected and the serum levels of cortisol.

A larger amount of serum can be collected and a lower serum levels of cortisol present at euthanasia fish with clove oil as compared with equipotent doses of MS222. Euthanasia with clove oil does not blunt the expected elevation of serum cortisol levels induced by acute stress premortem.

According to our findings, clove oil is a fast-acting agent which minimizes cortisol responses to euthanasia in zebrafish and allows the collection of a large volume of blood postmortem. These results represent a significant improvement in the method of euthanasia for zebrafish.

Effects of Clove Oil as a Euthanasia Agent on Blood Collection Efficiency and Serum Cortisol Levels in Danio rerio.
Effects of Clove Oil as a Euthanasia Agent on Blood Collection Efficiency and Serum Cortisol Levels in Danio rerio.

Serum C-reactive protein and white blood cell level as markers of successful percutaneous drainage of acute sterile peripancreatic fluid collection.

BACKGROUND
Percutaneous drainage is not a method of therapy is widely used recently to evacuate sterile peripancreatic fluid collections in patients with severe acute pancreatitis.However, many clinical studies have shown positive effects.

OBJECTIVE
We tested changes in laboratory parameters of serum C-reactive protein (CRP), complement factor 3-4 (C 3-4), tumor necrosis factor a (TNF-a), amylase, lipase and white blood cell (WBC) count in patients treated with percutaneous drainage.


METHOD
10 patients with severe acute pancreatitis with peripancreatic fluid collections were monitored.Laboratory parameters and the amount of fluid drained was measured on the 1st, 5th and 10th day. Statistical analysis was performed using Statistics for Windows (Version 7.0) software. P values ​​less than 0.05 were considered statistically significant.

RESULTS
We found a significant positive correlation between serum levels of CRP and WBC and drained fluid volume. We use this parameter as a marker of successful percutaneous drainage in the case of patients with severe acute pancreatitis complicated with sterile peripancreatic fluid.There no significant change in levels of C 3-4, tumor necrosis factor-Î + -, amylase and lipase.


CONCLUSION
Monitoring of serum CRP and WBC levels may be recommended for follow-up after percutaneous drainage of peripancreatic fluid.
BACKGROUND
CRP: C-reactive protein TNFÎ + -: Tumor Necrosis Factor a, C3-4: Complementary 3-4 WBC: White Blood Cells CT: Computed Tomography.

Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome.

Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome.

Background: Metabolomics is emerging as a valuable tool in the clinical sciences. However, one of the major challenges in clinical metabolomics is of limited use standardized guidelines for sample collection and handling. In this study, we conducted a pilot analysis of serum and plasma to determine the effects of processing time and collection tubes at the metabolome.

Methods: Blood was collected in three tubes: Vacutainer serum separator tube (SST, serum), EDTA (plasma) and P100 (plasma) and stored at 4 degrees for 0, 0.5, 1, 2, 4 and 24 hours prior to centrifugation. Compounds were extracted using liquid-liquid extraction to obtain a fraction of hydrophilic and hydrophobic and analyzed using liquid chromatography-mass spectrometry.

The difference between blood collection tubes and sample processing time is evaluated (ANOVA, Bonferroni FWER ≤ 0.05 and ANOVA, Benjamini Hochberg FDR ≤ 0.1, respectively). Results: Among the serum and plasma tubes 93.5% of the compound overlaps, 382 unique compounds for serum and one unique compound to the plasma. There are 46, 50 and 86 compounds is affected by the processing time in the SST, EDTA tube and P100, respectively, including many lipids.

In contrast, 496 242 hydrophilic and hydrophobic compounds different from a collection tube. Forty-five different chemical classes include alcohols, sugars, amino acids and lipids prenol influenced by the choice of blood collection tubes.

Conclusion: Our results indicate that the choice of blood collection tubes have a significant impact on the metabolites detected and their overall abundance. Perhaps surprisingly, the variation in sample processing time has less effect than the collection tube; However, a larger sample size is needed to confirm this.

Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome.
Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome.

Therapeutic antibiotic serum concentrations by two blood collection methods within the pediatric patient: A comparative effectiveness trial.

venipunctures repeatedly and fingersticks to confirm serum drug concentration causing pain and discontent for pediatric patients and their families. In many organizations, the standard of care to obtain therapeutic serum drug concentration by peripheral venipuncture or fingerstick capillary, even when the patient has any peripheral intravenous catheter (PIV) or a central venous catheter (CVC).

The main objective of this study was to assess the agreement between serum tobramycin / vancomycin concentrations were collected from CVC or PIV, compared to venipuncture or fingerstick. Among patients hospitalized children (aged 3 months to 22 years), 36 paired blood samples were collected.

Through random serum vancomycin and tobramycin concentrations were compared between samples of peripheral intravenous or CVC, and venipuncture or fingerstick samples in the same patient.

A rigorous sampling protocol to obtain samples followed, including sample collection CVC / PIV before venipuncture or fingerstick, less than 2 minutes between collections of samples from different sites, and based on the volume of waste flush and strict protocol. corresponding correlation coefficient showed a substantial agreement between the CVC / PIV and venipuncture / fingerstick concentration to vancomycin (n = 17) and tobramycin (n = 19) (Rc = 0.982 for both).

Bland-Altman analysis showed overall good agreement between methods in these subjects and minimal bias. As a result, use and waste protocols flush lumen volume based-, children with venipunctures catheter may not require additional and / or fingersticks confirmation of drug concentrations while hospitalized, improve service quality and patient satisfaction.

Switching from serum to plasma: Implementation of BD Vacutainer® Barricor™ Plasma Blood Collection Tubes improves sample quality and laboratory turnaround time.

Switching from serum to plasma: Implementation of BD Vacutainer® Barricor™ Plasma Blood Collection Tubes improves sample quality and laboratory turnaround time.

For blood, most standard 24/7 (immuno) chemical parameters measured either in serum or lithium-heparin plasma. Standard tube gel serum and plasma have their flaws when timely analysis required high quality results.

Serum require the freezing time and disruption of bubbles in the plasma gel and hydrophobic analyte adsorption into the gel layer is potentially harmful high-quality results from gel lithium heparin tube.

We sought to evaluate the impact of the BD Vacutainer® Barricor ™ Tube (Barricor ™) in laboratory efficiency by measuring its effect on TAT and quality of the samples, as well as evaluating opportunities potential costs resulting from an increase in the sample data extracted quality.

TAT and remediation activities and was arrested for two 6 phases of the moon. Serum is used as the dominant matrix of the first stage and Barricor ™ plasma used in the second phase.Barricor ™ significantly reduced the median TAT, especially for routine sample-priority during peak hours.

TAT key performance indicators (percentage yield available within 90 minutes) increased to> 90% for STAT and routine priority samples. Conversion of gel serum, Barricor ™ reduces fibrin-related remediation activities from 2.3% to 0.4%. This resulted in a reduction in remediation-related costs of € 6010.47 during the study period.

By apply Barricor ™, we see a significant reduction in TAT and reduction of fibrin-related remediation time and cost, when compared to the dominant serum workflow. TAT improved opens up the possibility of consolidation to a single priority level, eliminating the need to use STAT priority level.

Switching from serum to plasma: Implementation of BD Vacutainer® Barricor™ Plasma Blood Collection Tubes improves sample quality and laboratory turnaround time.
Switching from serum to plasma: Implementation of BD Vacutainer® Barricor™ Plasma Blood Collection Tubes improves sample quality and laboratory turnaround time.

Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in serum and heparin plasma samples.

For blood, most standard 24/7 (immuno) chemical parameters measured either in serum or lithium-heparin plasma. Standard tube gel serum and plasma have their flaws when timely analysis required high quality results. Serum require the freezing time and disruption of bubbles in the plasma gel and hydrophobic analyte adsorption into the gel layer is potentially harmful high-quality results from gel lithium heparin tube.

We sought to evaluate the impact of the BD Vacutainer® Barricor ™ Tube (Barricor ™) in laboratory efficiency by measuring its effect on TAT and quality of the samples, as well as evaluating opportunities potential costs resulting from an increase in the sample data extracted quality.

TAT and remediation activities and was arrested for two 6 phases of the moon. Serum is used as the dominant matrix of the first stage and Barricor ™ plasma used in the second phase.

Barricor ™ significantly reduced the median TAT, especially for routine sample-priority during peak hours. TAT key performance indicators (percentage yield available within 90 minutes) increased to> 90% for STAT and routine priority samples. Conversion of gel serum, Barricor ™ reduces fibrin-related remediation activities from 2.3% to 0.4%.

This resulted in a reduction in remediation-related costs of € 6010.47 during the study period.By apply Barricor ™, we see a significant reduction in TAT and reduction of fibrin-related remediation time and cost, when compared to the dominant serum workflow. TAT improved opens up the possibility of consolidation to a single priority level, eliminating the need to use STAT priority level.